Fig. 1.

Markers of ferroptosis in human, ALS-affected spinal cord. a, b Quantitative in situ mapping of iron in transverse sections of human spinal cord reveals an overall increase in iron in the ALS-affected grey matter. c Ferroxidase activity in human, ALS-affected spinal cord. d, e Transcripts associated with iron handling in human, ALS-affected spinal cord tissue. f-h Biochemical markers of ferroptosis (glutathione, lipid peroxidation, and ALOX5 protein levels) in human, ALS-affected spinal cord. i, j Transcripts associated with ferroptosis in human, ALS-affected spinal cord tissue. Data in g and h are expressed relative to controls. Data points (b, c, e-h, j) represent individual control or ALS cases. Values in transcript heatmaps (d, i) represent z-scores for individual control and ALS cases. Violin plots in e, j represent overall transcript signature for features indicated, derived from heatmap data shown in d, i, respectively. Crosses in heatmaps represent excluded samples. P values show significant differences where indicated. Error margins in bar graphs are S.E.M. Solid lines in violin plots represent median, dotted lines represent 25^th/75^th percentiles, truncated at min-max values