Fig. 4.
Markers of glial activation and ferroptosis are correlated in human, ALS affected spinal cord. a Protein levels of neurotoxic astrocyte marker C3 expressed relative to controls. b, c Transcripts associated with glial activation, highlighting selected markers designated for pan, A1 and A2 activation. Genes associated with neurotoxicity in vitro (shown in Supplementary Fig. 7) are indicated by ‘a’. Violin plots in c represent overall transcript signature for features indicated, derived from heatmap data shown in b, including genes associated with neurotoxicity in vitro (Neurotoxic). d Correlation between overall transcript signatures for ferroptosis and glial activation associated with neurotoxicity. Symbols represent composite z-scores for ferroptosis (from Fig. 1j) and glial activation associated with neurotoxicity (from c) for corresponding individual cases. P values show significant differences where indicated (a, c) or significance of correlation (d). Data points in a, c, d represent individual control or ALS cases. Values in transcript heatmap (c) represent z-scores for individual control and ALS cases. Crosses represent excluded samples. Error margins are S.E.M. per mean (a) or per case (d), or 95% confidence interval of linear regression (dashed lines) in d. Solid lines in violin plots represent median, dotted lines represent 25th/75th percentiles, truncated at min-max values