Table I. Meiotic progression of wild-type (WT) and TR–/– G4 mouse oocytesa.
9 h of culture |
12–14 h of culture |
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Oocyte | Number examined | Stage of meiosisb |
Number examined | Stage of meiosisb |
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GV–GVBD | MI (%) | AI–TI | GV–GVBD | MI | AI–TI | MII (%) | |||
WT | 39 | 1 | 31 (79%) | 7 | 28 | 0 | 3 | 0 | 25 (89%) |
1 misaligned | 0 misaligned | ||||||||
TR–/– G4 | 17 | 6 | 6 (35%) | 5 | 21 | 5 | 3 | 1 | 12 (57%) |
4 misaligned | 8 misaligned |
aDetermined by immunofluorescence staining and confocal microscope.
bGV, germinal vesicle; GVBD, germinal vesicle breakdown; MI, metaphase of meiosis I; AI, anaphase I; TI, telophase I; MII, metaphase of meiosis II.