Fig. 4. Lysines 5 and 12 are deacetylated on repressed DHFR promoter. Chromatin was extracted from G₁ (4 h) or G₁–S (10 h) cells, and immunoprecipitated with anti-AcH4 or anti-acetylated H4 K5, K8, K12 or K16 antibodies, as indicated. The results are expressed as fold variation with reference to the G₁ cells (mean values from three determinations in three independent experiments, with range bars indicating standard deviations). (A) DHFR; (B) GAPDH.