Fig. 1. Readthrough visualization. Different readthrough motifs, ranging from 1 to 30%, were introduced in the ADE2 gene cloned into a pFL38 plasmid. These plasmids were used to transform the FS1 strain. Exponentially growing cells were spotted on plates (1 × 10⁶ cells/ml) and colour was checked after 3 days at 30°C.