Table III. Nucleotides +7, +8 and +9 have a 10-fold effect on readthrough.
Sequencea | Readthrough efficiency (%) | Vector name | ||||||
---|---|---|---|---|---|---|---|---|
+1 | +4 | +7 | +10 | |||||
GCA | ACA | CAA | TAG | CAA | TTA | CAG | 30 | (pAC-TMV) |
G | T | Q | * | Q | L | Q | ||
--- | --- | --- | --- | --- | GAA | --- | 3 | (pAC-GAA) |
E | ||||||||
--- | --- | --- | --- | --- | --- | TAT | 14 | (pAC-TAT) |
Y | ||||||||
AAA | CCA | CAA | TAG | CAA | GAA | TAT | 2 | (pAC-CAAP) |
K | P | Q | * | Q | E | Y | ||
--- | --- | --- | --- | --- | TTA | --- | 22 | (pAC-TTA) |
L |
aNucleotide position (relative to the first base of the stop codon) is indicated.