Table V. The effect of the 3′ sequence is not correlated to properties of tRNAUAA.
| Sequence | Readthrough efficiency (%)a |
Vector name | |||||||
|---|---|---|---|---|---|---|---|---|---|
| –tRNAAAGLeu | +tRNAAAGLeu | ||||||||
| +1 | +4 | +7 | +10 | ||||||
| GGA | ACA | CAA | TAG | CAA | TTA | CAG | 30 | ND | (pAC-TMV) |
| G | T | Q | * | Q | L | Q | |||
| --- | --- | --- | --- | --- | CTT | --- | 3.5 | ND | (pAC-CTT) |
| L | |||||||||
| ACG | ACG | ATA | TAA | CAA | CTT | AAA | 0.8 | 0.9 | (pAC-CRc)b |
| T | T | I | * | Q | L | K | |||
| --- | --- | --- | --- | --- | TTA | --- | 9 | 9.1 | (pAC-CRctta) |
aThe UUA anticodon of tRNAUAALeu was modified to AAG. This new tRNAAAG is able to recognize the CUU codon and is functional in yeast (data not shown). The expression of tRNAAAG does not reproduce the effect of the replacement of CUU by the UUA triplet.
bThis sequence has been identified by O. Namy and J.-P. Rousset (unpublished).