Table 3.

Matrix effects of the method.

	3-MT			MN			NMN
	Theoretical concentration (ng/mL)	Measured concentration (ng/mL)	Bias (%)	Theoretical concentration (ng/mL)	Measured concentration (ng/mL)	Bias (%)	Theoretical concentration (ng/mL)	Measured concentration (ng/mL)	Bias (%)
L 1:1	15.92 ± 4.32	15.70 ± 4.63	−1.42	9.08 ± 4.81	9.05 ± 5.29	−0.21	10.16 ± 3.69	10.46 ± 4.05	2.91
L 4:1	23.67 ± 6.91	24.19 ± 7.81	2.18	12.72 ± 7.70	13.71 ± 9.30	7.76	14.46 ± 5.90	14.42 ± 6.30	−0.26
L 1:4	8.17 ± 1.73	7.98 ± 2.05	−2.28	5.43 ± 1.92	5.22 ± 2.21	−3.78	5.86 ± 1.48	5.82 ± 1.83	−0.69
H 1:1	414.42 ± 4.32	386.48 ± 20.85	−6.74	407.58 ± 4.81	399.10 ± 32.27	−2.08	408.66 ± 3.69	391.32 ± 26.51	−4.24
H 4; 1	183.07 ± 6.91	167.34 ± 22.10	−8.59	172.12 ± 7.70	158.06 ± 16.05	−8.17	173.86 ± 5.90	160.99 ± 12.45	−7.40
H 1:4	645.77 ± 1.73	619.20 ± 26.18	−4.11	643.03 ± 1.92	629.97 ± 26.29	−2.03	643.46 ± 1.48	638.69 ± 15.80	−0.74

Data are expressed as Mean ± SEM.