Figure 3.

Genetic regional intolerance to variation of GRID2. The 3D MTR analysis for GRID2 was developed from the GluD1 structure described by Burada et al. (2020) with 3D MTR residue window of 31 (see Supplementary Data 1 and 2). Low 3D MTR scores (blue) indicate residues less tolerant to variation, while high scores [81] indicate residues that are more tolerant to variation. (A₁) Full view of GRID2 3D MTR scores mapped onto the GluD1 protein structure, assuming that GluD2 has a similar architecture to GluD1. (A₂) Top down view of the amino-terminal domain (NTD) of GRID2 3D MTR scores on the GluD1 protein structure. (B_1-2) Isolated subunit GRID2 3D MTR scores shown with ribbon and space fill model. (C) GRID2 variants clinically relevant or tested here that are not present in gnomAD v2.1.1 from Supplementary Tables S3 and S5 shown in context of GRID2 3D MTR scores.