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. 2023 Nov 7;33(4):355–373. doi: 10.1093/hmg/ddad188

Figure 5.

Figure 5

GluD1-R341Q reduces binding between GluD1-NTD and Cbln2. (A) Schematic of the biomembrane force probe (BFP) experiment. (B) Photomicrograph of the BFP experiment. (C) Force trace of a single BFP trial where there was no GluD1/Cbln2 interaction, as demonstrated by the lack of a tension force (+) upon probe retraction. (D) Force trace of a single BFP trial where there was a GluD1/Cbln2 interaction as evidence by the positive force reading upon probe retraction. (E) Adhesion frequencies between HEK293T cells expressing similar levels of GluD1 WT or R341Q (Supplementary Fig. S5) and BFP beads coated with indicated concentrations of Cbln2. Each point represents an adhesion frequency evaluated from the observed number of binding events divided by the total number of repeated contacts (50-100) between a single pair of cell and bead. Also shown are mean ± SEM for each condition. Abbreviations: RBC, red blood cell; SA, streptavidin. Means were compared with an unpaired ANOVA with post hoc Tukey test, where **** indicates P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Only pertinent significant comparisons are shown.