Table 2.
Screen for constitutive activity of GRID1 and GRID2 human variants.
| Constitutive activity (NMDG+− Baseline) nA (n) |
d-Serine effecta (Baseline −10 mM d-Serine) nA (n) |
Pentamidine effecta (Baseline −100 μM Pentamidine) nA (n) |
|
|---|---|---|---|
| GluD1-WT | 200 ± 60 (13) | 2.3 ± 2.9 (13) | 37 ± 11 (14) |
| GluD1-R341Q | 230 ± 130 (6) | 7.2 ± 1.8 (6) | 64 ± 15 (6) |
| GluD1-S647F | 190 ± 100 (10) | 14 ± 3.8 (10) | −47 ± 11 (10) |
| GluD1-T649I | 140 ± 76 (9) | 8.9 ± 1.2 (9) | −45 ± 9.5 (9) |
| GluD1-A650T | 2220 ± 410 (12)* | 810 ± 130 (12)* | −1090 ± 200 (12)* |
| GluD1-N651S | 100 ± 43 (10) | 7.6 ± 1.6 (10) | 38 ± 8.3 (10) |
| GluD2-WT | 18 ± 1.6 (14) | −3.6 ± 1.0 (14) | 3.6 ± 4.8 (14) |
| GluD2-D535E | 56 ± 23 (12) | −5.8 ± 2.4 (12) | −0.67 ± 1.1 (12) |
| GluD2-S646P | 28 ± 23 (11) | 5.2 ± 6.0 (11) | 6.5 ± 5.4 (11) |
| GluD2-T649A | 113 ± 14 (13) | −35 ± 5.6 (14) | −18 ± 4.8 (14) |
| GluD2-T649M b | 37 ± 11 (16) | −0.31 ± 2.8 (16) | 5.8 ± 7.0 (16) |
| GluD2-A654D | 740 ± 110 (12)* | −29 ± 10 (14) | −700 ± 160 (14) |
| GluD2-A654T c | 1300 ± 160 (14)* | −1000 ± 130 (14)* | −930 ± 130 (14)* |
| GluD2-L656V | 1040 ± 140 (12)* | −460 ± 53 (12)* | −640 ± 190 (12)* |
| GluD2-R710W | 27.7 ± 2.3 (11) | −2.3 ± 0.60 (11) | 0.82 ± 1.9 (11) |
Data are mean ± SEM (n).
*Indicates P < 0.05, One way ANOVA, Dunnett’s multiple comparisons test.
aPositive values indicate an increase of activity and negative values indicate a decrease of activity.
bThis was found in gnomAD.
cThis is the constitutively active lurcher mutation.