Table 3.
Surface expression of GRID1 and GRID2 variants determined by β-lactamase activity.
| Constitutive activity | Surface/Total, %WT (n) | Total, %WT (n) | |
|---|---|---|---|
| GluD1-WT | — | 100 ± 4.7 (8) | 100 ± 15 (8) |
| GluD1-R341Q | — | 108 ± 6.6 (5) | 75 ± 16 (5) |
| GluD1-S647F | — | 103 ± 5.6 (9) | 89 ± 8.0 (9) |
| GluD1-T649I | — | 86 ± 7.9 (9) | 89 ± 14 (9) |
| GluD1-A650T | present | 48 ± 12 (5)* | 33 ± 11 (5)* |
| GluD1-N651S | — | 102 ± 2.6 (6) | 90 ± 11 (6) |
| GluD2-WT | — | 100 ± 6.4 (21) | 100 ± 11 (21) |
| GluD2-D535E | — | 95 ± 9.9 (8) | 63 ± 16 (8)* |
| GluD2-S646P | — | 29 ± 9.3 (3)* | 1.9 ± 2.6 (3)* |
| GluD2-T649A | likely | 109 ± 11 (9) | 92 ± 9.3 (9) |
| GluD2-T649M | — | 84 ± 10 (9) | 57 ± 14 (9)* |
| GluD2-A654D | present | 35 ± 7.5 (8)* | 14 ± 4.2 (8)* |
| GluD2-A654T | present | 109 ± 7.5 (11) | 78 ± 18 (11) |
| GluD2-L656V | present | 99 ± 6.9 (7) | 115 ± 8.4 (7) |
| GluD2-R710W | — | 111 ± 7.2 (10) | 93 ± 7.0 (10) |
Data are mean ± SEM (n). See Fig. 6 for related graph.
* P < 0.05 one way ANOVA, with Dunnett’s multiple comparisons test, all variants compared to WT (normalized to 100%).