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. 2023 Aug 19;14(2):854–868. doi: 10.1016/j.apsb.2023.08.014

Figure 3.

Figure 3

Increased PDL-1 expression and promoted apoptosis of ID8 cells. (A) Expression of PDL-1 on ID8 cells was detected by flow cytometry. The supernatant from transfected cells was collected to stimulate lymphocytes, and then the lymphocytes culture medium was harvested to incubate tumor cells. The expression of PDL-1 on ID8 cells in each treatment group was analyzed using flow cytometry. Data are presented as mean ± SEM (n = 3). ∗∗∗∗P < 0.0001. (B) Apoptosis of ID8 cells was detected by flow cytometry. We transfected the CFSE-labeled tumor cells with different complexeses and added lymphocytes for co-incubation for 48 h. The apoptosis of tumor cells using PE-Annexin V staining method was subsequently analyzed. Data are presented as mean ± SEM (n = 3). ∗∗∗∗P < 0.0001.