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. 2023 Aug 19;14(2):854–868. doi: 10.1016/j.apsb.2023.08.014

Figure 4.

Figure 4

Polarization of macrophages in vitro. (A) After being stimulated by the supernatants from transfected ID8 cells for 48 h, the polarization status of macrophages in vitro was analyzed by flow cytometry. The secretion levels of TNF-α (B) and IFN-γ (C) from stimulated macrophages were measured by ELISA. (D) The mRNA levels of immunosuppressive M2 phenotype-related genes (left) and proinflammatory M1 phenotype-related genes (right) in stimulated macrophages were detected by RT-PCR. Data are presented as mean ± SEM (n = 3). ∗P < 0.05; ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. (E) Protein levels in the macrophages stimulated by supernatants from F-DPC/pvax, F-DPC/pIL-12, or F-DPC/pIL-12/iPDL-1 transfected ID8 cells were analyzed by Western blot.