Fig. 2. PAQ-monoclonal antibody depletes the majority of Mediator components from nuclear extracts. Four per cent of input (IN-JNE), 4.4% of PAQ depleted (ΔPAQ) and mock depleted (Δcontrol) Jurkat nuclear extract together with 20% of the PAQ (E-PAQ)- or the mock precipitated (E-control) material were analyzed by western blotting. Cross-reactions of secondary antibodies with IgG-HC and IgG-LC are marked (*).