Table 3.
Amino acids at the binding pocket and type of bonds involved. Different polymorphisms of AChE2 result in different amino acid compositions in the binding pocket, which in turn results indifferent types of bonds with diazoxon ligand.
| Strain/Isolate | Binding pocket | Hydrogen bonds | Hydrophobic bonds | Ionic bonds |
|---|---|---|---|---|
| Susceptible | F107, S108, W114, G150, E154 L161, W229, N359, I360,T363, R366, H476, D478, G477 | E154-G150 | W114-L-161 | E154-Diazoxon |
| R366-F107 | W114-W229 | |||
| N359-T363 | I159-L161 | |||
| T363-I360 | F107-T363 | |||
| H476-N359 | W114-Diazoxon | |||
| G150-E154 | I360-Diazoxon | |||
| N-Diazoxon | W229-Diazoxon | |||
| T363-Diazoxon | ||||
| F107-Diazoxon | ||||
| C1 | V106, F107, S108, P109, R110, G149, G150, E154, G155, I159, L161, W229, N359, I360, T363 H476, L480 | H476-E356 | W229-Diazoxon | G154-Diazoxon |
| H476-N359 | I360-Diazoxon | R110-Diazoxon | ||
| T363-I360 | T363-Diazoxon | |||
| N359-T363 | P109-Diazoxon | |||
| T364-I360 | L161-Diazoxon | |||
| Y362-S358 | I159-Diazoxon | |||
| L361-G357 | ||||
| Y355-S368 | ||||
| G357-L361 | ||||
| S358-Y362 | ||||
| I360-T364 | ||||
| N359-Diazoxon | ||||
| C2 | V106, F107, S108, P109, R110, Y117, G149, G150, E154, G155, I159, L161, W229, N359, I360, T363, H476, G477, L480 | H476-N359 | F107-T363 | H476-Diazoxon |
| N359-T363 | I159-L161 | N359-Diazoxon | ||
| I360-T363 | F107-Diazoxon | R110-Diazoxon | ||
| Y362-S358 | W229-Diazoxon | |||
| G357-L361 | V106-Diazoxon | |||
| H476-E356 | L161-Diazoxon | |||
| F472-S368 | I159-Diazoxon | |||
| N355-S358 | R110-Diazoxon | |||
| D359-Diazoxon | T363-Diazoxon | |||
| R110-Diazoxon |