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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Ann Neurol. 2023 Sep 14;94(6):1048–1066. doi: 10.1002/ana.26770

Fig. 3.

Fig. 3.

DM accumulate lipid droplets visualized with PLIN2. (A) Detail of a DM triple-labeled for Iba1 (blue), FTL (red) and PLIN2 (green). Note the extensive cytoplasmic labeling (arrowheads) of PLIN2 visualized in the merged image of FTL and PLIN2. Scale bars, 30 μm. (B) The density and percentage of Iba1+FTL+PLIN2+DM were similar in CMI and nCMI cases. (C and D) The density of lipid droplets containing DM (Iba1+FTL+PLIN2+; black) was significantly associated with total microglia in white matter lesions (r2=0.6315; p=0.0105). Nondegenerative microglia that lacked PLIN2 (Iba1+FTL−, salmon) and DM that lacked PLIN2 (Iba1+FTL+; blue) were not significantly associated with the lesion burden of microgliosis. (E) Detail of an Iba1+ (blue), PLIN2+ (green) microglial cell with lipid peroxidation injury visualized by staining for 4-hydroxynonenol (4-HNE; red). Orthogonal view at right. Scale bars, 30 μm.