TABLE 1.

Effects of various treatments on the incorporation of adenine into host and C. trachomatis DNAs

Cell linea	Temp (°C)	Addition(s)b	Incorporation of [3H]adenine into DNA (103 dpm/106 cells)c
			Mock infected	C. trachomatis infected
CHO K1	33		2.1 ± 0.8	163 ± 11.9
CHO K1	40		1.8 ± 1.0	111 ± 10.4
CHO 58	33		1.9 ± 0.6	153 ± 9.4
CHO 58	40		0.9 ± 0.5	102 ± 9.8
Mouse L	37		2.8 ± 1.1	147 ± 13.6
Mouse L	37	Fumonisin B1	3.1 ± 1.3	154 ± 14.9
Mouse L	37	Brefeldin A	2.4 ± 0.9	139 ± 9.4
Mouse L	37	Sphingomyelinase	2.9 ± 1.3	144 ± 11.5
Mouse L	37	Brefeldin A + sphingomyelinase	3.3 ± 1.6	137 ± 12.3

^aTriplicate cultures of wild-type CHO K1, CDP-choline synthetase mutant CHO 58, and wild-type mouse L 929 cells were seeded, cultured, and infected with C. trachomatis L2 or left as mock-infected controls as described in Materials and Methods and the report of McClarty and Tipples (25). 

^bFor mock-infected and C. trachomatis-infected cultures treated with brefeldin A (5 μg ml⁻¹), fumonisin B₁ (20 μM), and/or sphingomyelinase (0.05 U ml⁻¹), the inhibitors were added at the start of the infection (2 h p.i.). 

^cIncorporation of radiolabelled adenine into host cell or C. trachomatis L2 DNA was determined after addition of radiolabel at 22 h p.i. and continuing incubation for 3 h. Cells were harvested and processed as described previously (25). Results are expressed as means ± standard deviations of results from three separate experiments.