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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Mol Genet Metab. 2023 Aug 15;140(3):107686. doi: 10.1016/j.ymgme.2023.107686

Figure 6. Purine supplementation restores fertility and fecundity to adsl-1.

Figure 6.

Supplementation with 10 mM adenosine (Ad), or guanosine (Gu) restores fertility (A) and fecundity (B) of adsl-1(RNAi) animals. Supplementation with drugs to interfere with DNPS, including 22 μM methotrexate or 29 nM lometrexol, had no significant effect on (C) fertility or (D) fecundity of adsl-1(RNAi) animals. The controls for methotrexate and lometrexol are 8 μL and 155 μL of DMSO, respectively. Evidence for the uptake and inhibitory effect of methotrexate and lomotrexol is in Fig. 7. Each dot represents the value from an RNAi experiment with between 9 and 20 animals. Error bars indicate S.D. *, **, and **** represent p<0.05, p<0.01, and p<0.0001, respectively. Significance was calculated using ANOVA with Šidák’s multiple comparison test.