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. Author manuscript; available in PMC: 2024 Jul 3.
Published in final edited form as: Mol Cancer Ther. 2024 Jan 3;23(1):56–67. doi: 10.1158/1535-7163.MCT-21-0783

Figure 2. Cx26 cytosolic tail peptide binds both FAK and NANOG and penetrates the cell membrane upon addition of an antennapedia (Antp) leader sequence.

Figure 2.

A) Table denoting dissociation constants for interactions between Cx26 and NANOG and FAK. B) Cartoon depiction of complex based on SPR analysis. C) Summary table of FAK and NANOG dissociation constants with Antp alone, tagged cytosolic tail, or tagged cleaved tail peptides by SPR analysis. D) Intracellular flow cytometry staining and E) immunofluorescence staining for Antp following 10 μM aCx26-pep treatment for 16 h, demonstrating the ability of the peptide to cross the plasma membrane. Scale bar, 40 μm.