| Antibodies |
| Anti-Digoxigenin-AP, Fab fragments |
MilliporeSigma |
11093274910 |
| Chemicals, peptides, and recombinant proteins |
| 2×Super Pfx MasterMix |
Cowin Biotech |
CW2965F |
| Gateway™ LR Clonase™ II Enzyme mix |
ThermoFisher Scientific |
11791020 |
| KAPA2G Fast HotStart ReadyMix |
KAPA Biosystems |
7961235001 |
| AccuStart II DNA polymerase |
Quantabio |
95141 |
| PowerSYBR Green PCR Master Mix |
ThermoFisher Scientific |
4367659 |
| SuperScript™ III First-Strand Synthesis SuperMix |
ThermoFisher Scientific |
18080400 |
| TURBO™ DNase |
ThermoFisher Scientific |
AM2238 |
| PACE 2x Genotyping Master Mix High ROX 500nM |
3Cr Biosciences |
001-0010 |
| ISceI |
New England Biolabs |
R0694 |
| ClaI |
New England Biolabs |
R0197 |
| SacII |
New England Biolabs |
R0157 |
| XhoI |
New England Biolabs |
R0146 |
| NotI-HF |
New England Biolabs |
R3189 |
| HindIII-HF |
New England Biolabs |
R3104 |
| EcoRI-HF |
New England Biolabs |
R3101 |
| ScaI-HF |
New England Biolabs |
R3122 |
| BstEII-HF |
New England Biolabs |
R3162 |
| NcoI-HF |
New England Biolabs |
R3193 |
| PacI |
New England Biolabs |
R0547 |
| SspI-HF |
New England Biolabs |
R0132 |
| BbsI-HF |
New England Biolabs |
R3539 |
| BclI-HF |
New England Biolabs |
R3160 |
| MscI |
New England Biolabs |
R0534 |
| NsiI-HF |
New England Biolabs |
R3127 |
| BsrGI-HF |
New England Biolabs |
R3575 |
| BamHI-HF |
New England Biolabs |
R3136 |
| SalI-HF |
New England Biolabs |
R3138 |
| (Z)-4-Hydroxytamoxifen |
MilliporeSigma |
H7904 |
| DIG-labeled DNA marker VII |
MilliporeSigma |
11669940910 |
| DIG Easy Hyb™ Granules |
MilliporeSigma |
11796895001 |
| DIG DNA Labeling Mix |
MilliporeSigma |
11277065910 |
| Casein Blocking Buffer 10x |
MilliporeSigma |
B6429 |
| CDP-Star®, ready-to-use |
MilliporeSigma |
12041677001 |
| Qtracker 705 vascular labels |
ThermoFisher Scientific |
Q21061MP |
| TRIzol™ Reagent |
ThermoFisher Scientific |
15596026 |
| SpCas9 |
Wolfe Lab |
N/A |
| LbCas12a-2xNLS |
Wolfe Lab |
N/A |
| Critical commercial assays |
| pGEM®-T Vector Systems |
Promega |
A3600 |
| NEBuilder® HiFi DNA Assembly Cloning Kit |
New England Biolabs |
E5520S |
| mMESSAGE mMACHINE™ SP6 Transcription Kit |
ThermoFisher Scientific |
AM1340 |
| MEGAscript™ T7 Transcription Kit |
ThermoFisher Scientific |
AM1334 |
| Blood & Cell culture DNA Midi kit |
QIAGEN |
13343 |
| NucleoSpin Gel and PCR Clean-up kit |
Takara Bio |
740609 |
| MultiSite Gateway Three Fragment Vector Construction kit |
ThermoFisher Scientific |
12537-023 |
| AllPrep DNA/RNA Micro Kit |
QIAGEN |
80284 |
| CloneJET PCR Cloning Kit |
ThermoFisher Scientific |
K1231 |
| Zero Blunt™ TOPO™ PCR Cloning Kit |
ThermoFisher Scientific |
451245 |
| Experimental models: Organisms/strains |
| Zebrafish: gata2aum27
|
Zhu et al.24
|
ZDB-ALT-111201-1 |
| Zebrafish: foxc1ap162
|
Banerjee et al.30
|
ZDB-ALT-140721-2 |
| Zebrafish: Tg(ubi:CtoH2b-cherry)jh63
|
Zhang et al.40
|
ZDB-ALT-151007-31 |
| Zebrafish: gata2aum345 (aka gata2afl) |
this study |
um345 |
| Zebrafish: foxc1aum383 (aka foxc1afl) |
this study |
um383 |
| Zebrafish: rasa1aum414 (aka rasa1afl) |
this study |
um414 |
| Zebrafish: rasa1aum255
|
this study |
um255 |
| Zebrafish: rasa1aum290
|
this study |
um290 |
| Zebrafish: ruvbl1chb10 (aka ruvbl1fl) |
this study |
chb10 |
| Zebrafish: Tg(gata2aECE:iCre)um337
|
this study |
um337 |
| Oligonucleotides |
| See Supplementary Table 4 for all oligonucleotide sequences |
|
|
| Recombinant DNA |
| Plasmid: pKHR8 |
Hoshijima et al.17
|
RRID:Addgene_74625 |
| Plasmid: p3E-MCS1 |
Moore et al.52
|
RRID:Addgene_49004 |
| Plasmid: pmE-CreERT2
|
Mosimann et al.56
|
RRID:Addgene_27321 |
| Plasmid: pToneDest |
Shin et al.55
|
RRID:Addgene_67691 |
| Plasmid: pToneTP |
Shin et al.55
|
RRID:Addgene_67692 |
| Plasmid: p5E-6xgata2aECEbas |
Shin et al.55
|
RRID:Addgene_132968 |
| Plasmid: pCSDest2 |
Villefranc et al.53
|
RRID:Addgene_22424 |
| Plasmid: pBSIce-gata2aKI |
this study |
N/A |
| Plasmid: pBSIsce-KI |
this study |
N/A |
| Plasmid: pCS-CreNS |
this study |
N/A |
| Plasmid: pCS-zfCre |
this study |
N/A |
| Plasmid: pDR274-gata2a_3pex5sgRNA1 |
this study |
N/A |
| Plasmid: pJET-mCherry |
this study |
N/A |
| Plasmid: pJET-mVenus |
this study |
N/A |
| Plasmid: pKHR8-foxc1aKI |
this study |
N/A |
| Plasmid: pKHR8-rasa1aKI |
this study |
N/A |
| Plasmid: pmE-CreNS |
this study |
N/A |
| Plasmid: pmE-MCS1 |
this study |
N/A |
| Plasmid: pmE-zfCre |
this study |
N/A |
| Plasmid: pmE-zfCreERT2
|
this study |
N/A |
| Plasmid: pTol1-gata2aECe:CreERT2;cryaa:mcherry |
this study |
N/A |
| Software and algorithms |
| ZEN2010 |
Zeiss |
RRID:SCR_014340 |
| MultiTime Series PLUS Macro |
Zeiss |
RRID:SCR_014340 |
| AxioVision SE64 Rel 4.9.1 software |
Zeiss |
RRID:SCR_002677 |
| Adobe Photoshop CC |
Adobe |
RRID:SCR_014199 |
| Adobe Premiere CC |
Adobe |
RRID:SCR_021315 |
| Adobe Illustrator CC |
Adobe |
RRID:SCR_010279 |
| ImageJ2 version 2.3.0/1.53q with FIJI |
NIH |
RRID:SCR_002285 |
| IC Capture 2.5 software |
IMAGINGSOURCE |
https://www.theimagingsource.com/enus/support/download/iccapture-2.5.1525.3931/
|
| Prism |
GraphPad Software |
RRID:SCR_002798 |
| R Package for Statistical Computing |
www.r-project.org
|
RRID:SCR_001905 |
| Other |
| Scanning Electron microscope |
Hitachi High-Technology Corp |
SU8010 |
| 710NLO confocal/two-photon microscope |
Zeiss |
RRID:SCR_017377 |
| Leica Fluorescence Dissection microscope |
Leica |
M165FC |
| Digital camera, AxioCam |
Zeiss |
MRc5 |
| Axioskop2 Plus |
Zeiss |
N/A |
| Imaging source USB CCD B&W 2.0 camera |
IMAGINGSOURCE |
DMK21AU04 |
| Spectrolinker™ UV Crosslinker |
Spectronics Corp |
XL-1000 |
| hybridization incubator |
Robbins Scientific |
Model 400 |
| ChemiDoc |
BIO-RAD |
12003153 |
| StepOnePlus Real-Time PCR Systems |
ThermoFisher Scientific |
RRID:SCR_015805 |
| Nylon Membrane, positively charged |
MilliporeSigma |
11209299001 |
| Hybridization Bags |
Cosmo Bio Co |
SE-S-1002-EX |
