TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant genotype, phenotype, and/or characteristics | Reference |
|---|---|---|
| S. typhimurium χ4550 | SR-11 pStSR101+gyrA1816 Δcrp-1 ΔasdA1 Δ(zhf-4::Tn10) Δcya-1 | 34 |
| E. coli | ||
| χ6212 | Asd− DH5α derivative | 27 |
| DH1 | Host strain for pJY4347 | 38, 45 |
| S. pneumoniae WU2 | Encapsulated type 3; virulent | 4 |
| Plasmids | ||
| pJY 4347 | Harbors 2.0-kb HindIII-KpnI fragment encoding the entire PspA+ (65 kDa)a cloned into HindIII-KpnI sites of pJY4313 | 44, 45 |
| pYA 3148 | Asd+ vector, high copy number (PUC-based Kmr) | 33 |
| pYA 3193 | Recombinant Asd+ vector harboring the C-terminally truncated pspA gene (1.5 kb); specifies 55-kDa PspA protein | This study |
| pYA 232 | Low-copy-number (pSC101 ori) plasmid containing lacIq repressor gene; Tcr | 27 |
a
The apparent molecular mass of a PspA monomer on SDS gels is 85 kDa.