Figure 2.

C. rodentium T2SS is necessary for successful infection in C57BL/6J mice. (a) C. rodentium shedding was assessed by CFU enumeration from feces shed daily post- infection and normalized to fecal weight. (b) Mucosal and lumenal sub-populations of C. rodentium in the cecum, ileum, and colon were measured by plating either the lumenal contents or the mucosal tissues on MacConkey agar and counting colony-forming units (CFU). At least n = 5 mice per group, Wilcoxon signed-rank test. (c,d) Hyperplasia was determined by either measuring the crypt length in (C) hematoxylin and eosin (H&E) stained murine intestinal samples or (D) by calculating the proportion of Ki67-positive epithelial (CDH1-positive) cells (as determined by immunofluorescent staining). There were at least n = 5 mice per group and a minimum of 30 crypts were measured per H&E-stained tissue section. (e,f) Systemic spread was assessed by measuring spleen colonization (E) and spleen weight (splenomegaly) (F). * - p < .05, ** - p < .01, *** - p < .001, **** - p < .0001. Wilcoxon signed-rank test.