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Fig. 4. APC/Fizzy-Related induces in vivo degradation of endogenous Aurora-A and Fizzy. Oocytes were microinjected with Fizzy-Related mRNA. Homogenates were prepared from individual oocytes hourly and analysed by coupled western blotting in the same nitrocellulose membrane for endogenous Aurora-A and Fizzy-Related. Fizzy degradation was followed by western blotting on a separate membrane.
