Figure 2. pIgR-deficient mice develop more steatohepatitis after chronic-binge ethanol feeding.

A) Schematic of chronic-binge ethanol feeding study in female and male pIgR^−/− mice (red) and wildtype littermates (grey).

B) Fecal IgA levels at the end of the study. </p/> C) Plasma IgA levels at the end of the study. </p/> D) Liver to body weight ratio.

E) Plasma ALT levels.

F) Representative images showing H&E staining of liver sections.

G) Hepatic triglyceride content.

H) Quantification of immunohistochemical staining for lipids using Oil Red O.

I) Representative images showing Oil Red O staining of liver sections.

J-M) mRNA levels of indicated genes (Cxcl1, Cxcl2, Ccr2, Cd11b) in livers of ethanol-fed mice as shown in Figure 2A, assessed by qPCR. Data are shown relative to the respective wildtype mice and normalized to 18S.

N) Quantification of immunohistochemical staining for infiltrating macrophages and neutrophils in the liver using Mac-1.

O) Representative images showing Mac-1 staining of liver sections.

Data shown as mean ± SEM of n=12–18/group from 6 independent experiments. * indicates p≤0.05, ** p≤0.01, *** p≤0.001. Exact p-values are provided in Table S2.