Figure 8. Copper chelators and p38 inhibitor reduce copper toxicity in cua-1(knu790[H828Q]) C.elegans strain.

A, B. Wild-type (WT) and mutant (H828Q) animals were grown for 3 days on plates, which were supplemented with 100 μM CuCl₂ alone or combined with either 100 μM BCS or 50 μM TTM. The plates were analyzed under the stereomicroscope (A) to quantify number of adults in the population (B). Both micrograms (A) and quantification (B) indicate that both BCS (50μM and 100mM) and TTM (50μM) reduced impact of Cu on the development of mutant animals(** p<0.01, **** p<0.0001; two way ANOVA; n=3 experiments). C. Wild-type (WT) and mutant (H828Q) animals were grown for 62h on plates, which were supplemented with 100 μM CuCl₂ only or in combination with inhibitor of p38 MAP kinases SB202190 at 5 or 10 μM concentration. The plates were analyzed to quantify number of adults in the population. Quantification shows that p38 inhibitor improves development of mutant animals grown in the presence of Cu (** p<0.01, *** p<0.001; one way ANOVA; n=3 experiments). Scale bar: 1.2 mm (A).