FIG. 2.

(Left) Time-lapse video phase microscopy images of Listeria migrating in NIH 3T3 C5 fibroblasts expressing 2.25-fold-increased levels of gelsolin (left panels) and in NIH 3T3 control fibroblasts with a normal gelsolin level (right panels). Images were taken at 60-s intervals. The white triangles represent reference points drawn with fixed granules within the cytoplasm of each cell. A black arrow points to a rapidly moving bacterium within a C5 cell. The images also show other bacteria moving at rapid velocities within the cell. The images in the right panels show two bacteria moving slowly within a control cell. Bar, 10 μm. (Right) Graphic depiction of bacterial velocities over time. Open squares indicate the velocities of the bacterium designated by the black arrow in Fig. 2, left. Closed squares indicate the velocities of the bacterium seen in the upper-left-hand corner of the control cell panels in Fig. 2, left.