Fig. 2. Peptide fragments and deduced amino acid alignment with atToc75-V. Purified outer envelope membranes from pea chloroplasts were separated by SDS–PAGE, and the protein band present at ∼66 kDa (indicated as psToc75-V in Figure 1B) was used for amino acid determination. Lane 1 shows peptides deduced by mass spectrometry. Lane 3 shows peptide sequences determined by Edman degradation. Lane 2 shows the amino acid sequence of atToc75-V deduced from the EST clones AF 360205, AY 040053 and at5g19620 that had the highest homology to the peptides determined from pea. The predicted presequence is written in italics. The peptide that is missing in at5g19620 due to a computational splice error is underlined.