Site-Specific Protein Conjugates Incorporating Para-Azido-L-Phenylalanine for Cellular and In Vivo Imaging

. Author manuscript; available in PMC: 2024 Nov 1.

Published in final edited form as: Methods. 2023 Oct 5;219:95–101. doi: 10.1016/j.ymeth.2023.10.001

Denaturation: 98°C for 30 seconds
Annealing: 25 cycles,	98°C for 10 seconds
	60°C for 30 seconds (αPD-L1) or 67°C for 30 seconds (PTEN)
	72°C for 4 minutes
Extension: 72°C for 2 minutes