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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Cell. 2023 Nov 27;186(25):5569–5586.e21. doi: 10.1016/j.cell.2023.10.024

Figure 3. Virome-wide TScan-II screen.

Figure 3

A) Schematic of the TScan-II human virome screen. The human virome library comprises 93,904 56aa fragments collectively tiled across the human virome (206 viral species, > 1,000 strains), including multiple HIV strains.

B) TScan-II screen of HIV elite controller F24 TCR with the virome library. Each dot represents one peptide, with the y-axis plotting the geometric mean of the fold-change across eight replicates against the HLA- DRB1*1101 allele. Red dots represent peptides with WT GAG epitope for this TCR; blue dots represent variant GAG epitopes, green dot represents a peptide from the HPV type 49.

C) TScan-II screen of a library of 12, 15, 18, and 20 aa fragments tiled at 1 AA intervals across the 56mer antigenic segments of HPV. The length of each dark blue indicates the scoring segment of HPV. Fragments in light blue did not score. The sequence depicted represents the minimal epitope.

D) FACS depicting reporter activation after a 6h co-culture with F24 T-cells. eHEK-293-II cells express the HLA-DRB1*1101 allele and a control peptide (left), HIV (middle), or HPV (right). Identical residues shared between the minimal HPV epitope and core GAG epitope are colored in red, while blue residues represent permitted amino acid substitutions based on Figure 2F.