Figure 5. Cancer-derived L. iners acquires additional genes for lactate production over NC-L. iners and alters cancer cell gene expression in pathways involved in intrinsic radiation sensitivity.

A. Overlapping and unique Genes on comparative genomic analysis for CC- L. iners (16%) vs NC-L. iners (2%) vs. shared (81%).

B. Overlapping genes on comparative genomic analysis for Healthy L. iners vs. dyplasia L. iners vs. CC- L. iners.

C. Sequential genes in pathways common to CC- L. iners and CC- L. iners (black) and unique to CC- L. iners (red) on comparative genomic analysis. LacG in CC- L. iners encodes the reversible enzyme, 6-phospho-beta-galactosidase, which converts lactose to galactose, while CC- L. iners ers utilizes only lactose in the lacDRA pathway. LacR is a repressor switch to turn off lactose metabolism to lactate.

D. Lollipop plot of metabolite pathways assignments for metabolites enriched in CC- L. iners isolates vs. NC-L. iners isolates. Galactose metabolism is the only upregulated pathway, consistent with lacG gene acquisition and 6-phospho-beta-galactosidase activity.

E. Top 7 differentially expressed metacore pathways for B1188 cells.

F. Top 7 differentially expressed metacore processes for B1188 cells.

G. Gamma H2AX and DAPI fluorescent staining of pretreated B1188 cells 30 hours after irradiation (8 Gy). Scale bars,100 μm

H. Gamma H2AX dynamics for primary cells B1188 treated with 8Gy in each CFS condition.

I. Radio-resistant EdU DNA synthesis assays for pretreated B1188 primary cells. Normalized to 0 Gy (black).

Log2 (Fold Change) cutoff of 2.0 (E,F); 1 independent experiment, 1 (I) or 3 (E) replicates, 2 CC-L. iners strains pooled (E) or separate (I); No statistical comparisons made (H,I).