KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Tyramide (TSA) linked Opal620	PerkinElmer	FP1495001KT
Cyanine 5 dyes	PerkinElmer	NEL745001KT
Neutral Buffer Formalin	EMS Diasum	15740-04
Syto 83	ThermoFisher	S11364
Critical commercial assays
Mm-Adora2a-C2	ACD	409438-C2
Mm-Drd1-C3	ACD	461908-C3
Mouse Whole Transcriptome Atlas RNA detection probes	Nanostring	GMX-RNA-NGSMsWTA-4
Buffer R	Nanostring	N/A
Buffer W	Nanostring	N/A
GeoMx Seq Code kit	Nanostring	GMX-NGS-SEQ
AmPure XP beads	Beckman Coulter	A63881
Multiplex 5X PCR Master Mix	Nanostring	N/A
Deposited data
Unprocessed (raw) data	This paper	https://doi.org/10.15139/S3/HS5BHE
Normalized data	This paper; supplement	N/A
In situ hybridization images	Allen Brain Atlas	https://mouse.brain-map.org/static/atlas
Experimental models: Organisms/strains
Mus Musculus C57BL/6J	The Jackson Laboratory	RRID: IMSR_JAX:000664
Mus Musculus DBA/2J	The Jackson Laboratory	RRID:IMSR_JAX:000671
Software and algorithms
ShinyGO v0.76 Web App	(Ge et al.)42	http://bioinformatics.sdstate.edu/go/
ComplexHeatmap v2.14.0 R package	(Gu et al.)43	https://doi.org/10.18129/B9.bioc.ComplexHeatmap
GO-Figure! Python scripts	(Reijnders and Waterhouse)44	https://gitlab.com/evogenlab/GO-Figure
GraphPad Prism v9.4.1	GraphPad Software, LLC	https://www.graphpad.com/
GeoMx Next Generation Sequencing Pipeline	Nanostring	N/A
GeoMx Digital Spatial Profiler Data Analysis Suite	Nanostring	N/A
SpatialDecon v1.8.0 R package	Nanostring	https://github.com/Nanostring-Biostats
Other
Bond RX Fully Automated Research Stainer	Leica Biosystems	N/A
Hyb EZ II hybridization oven	ACD	321710/321720
HybriSlip hybridization covers	Grace Bio-Labs	714022
Illumina NovaSeq	Novogene	N/A