KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE I-Ab Mycobacterium tuberculosis ESAT-64-1 tetramer | NIH Tetramer Core Facility | https://tetramer.yerkes.emory.edu/ |
| APC I-Ab Influenza A 2W1S tetramer | NIH Tetramer Core Facility | https://tetramer.yerkes.emory.edu/ |
| FITC anti-mouse CD45 | Biolegend | Cat# 147709; RRID:AB_2563542 |
| BUV395 anti-mouse CD4 | BD Biosciences | Cat# 563790; RRID:AB_2738426 |
| BV510 anti-mouse/human CD44 | Biolegend | Cat# 103043; RRID:AB_2561391 |
| PE anti-mouse P2X7 | Biolegend | Cat# 148703; RRID:AB_2650951 |
| BV786 anti-mouse P2X7 | BD Biosciences | Cat# 744724; RRID:AB_2742436 |
| BV711 anti-mouse/human KLRG1 | Biolegend | Cat# 138427; RRID:AB_2629721 |
| BV786 anti-mouse CX3CR1 | Biolegend | Cat# 149029; RRID:AB_2565938 |
| PE/Cyanine7 anti-mouse CD69 | Biolegend | Cat# 104511; RRID:AB_493565 |
| BV650 anti-mouse CXCR3 | Biolegend | Cat# 126531; RRID:AB_2563160 |
| PE anti-mouse/human Ki-67 | Biolegend | Cat# 151209; RRID:AB_2716014 |
| BV650 anti-mouse IFN-y | Biolegend | Cat# 505831; RRID:AB_11142685 |
| PE-CF594 anti-mouse FOXP3 | BD Biosciences | Cat# 562466; RRID:AB_11151905 |
| PerCP-Cyanine5.5 anti-mouse T-bet | Biolegend | Cat# 644805; RRID:AB_1595593 |
| BV421 anti-mouse CD45.1 | Tongo Biosciences | Cat# 75-0453-U025; RRID:AB_2621949 |
| APC anti-mouse CD45.2 | Biolegend | Cat# 109813; RRID:AB_389210 |
| Percp anti-mouse CD45.2 | Biolegend | Cat# 109825; RRID:AB_893351 |
| BV421 anti-mouse/human CD4 | BD Biosciences | Cat# 562425; RRID:AB_11154221 |
| APC-Cyanine7 anti-mouse/human CD44 | Biolegend | Cat# 103027; RRID:AB_830784 |
| PE anti-mouse-human CXCR3 | BioLegend | Cat# 353705; RRID:AB_10959652 |
| PE-Cyanine7 anti-human Ki-67 | BioLegend | Cat# 350525; RRID:AB_2562871 |
| Anti-mouse S100A9 | Bio-Techne | Cat# AF2065; RRID:AB_2184263 |
| PE anti-mouse CD4 | Biolegend | Cat# 100407; RRID:AB_312693 |
| FITC anti-rabbit antibody | BD Biosciences | Cat# 554020; RRID:AB_395212 |
| APC anti-mouse IFN-y | Biolegend | Cat# 505809; RRID:AB_315403 |
| InVivoMAb anti-mouse CD4 | BioXCell | Cat# BE0003-1; RRID:AB_1107636 |
| InVivoMAb anti-mouse CXCR3 (CD183) | BioXCell | Cat# BE0249; RRID:AB_2687730 |
| Anti-ARTC2.2 Treg-protector nanobodie | Biolegend | Cat# 149802; RRID:AB_2565485 |
| Pe/Cyanine5 anti-mouse CD69 | Biolegend | Cat# 104510; RRID:AB_313113 |
| BV785 anti-mouse/human CD44 | Biolegend | Cat# 103041; RRID:AB_11218802 |
| Bacterial and virus strains | ||
| Mycobacterium bovis MP287/03 | José Soares Ferreira Neto, PhD | Grown in house |
| Mycobacterium tuberculosis H37Rv | American Type Culture Collection | Grown in house |
| Influenza A PR8/34 | Jie Sun, PhD | Grown in house |
| Influenza A PR8/2W | Ryan Langlois, PhD | Grown in house |
| Chemicals, peptides, and recombinant proteins | ||
| Middlebrook 7H9 medium | Becton Dickinson | Cat# 271310 |
| Middlebrook 7H10 Agar | Becton Dickinson | Cat# 262710 |
| Middlebrook ADC Growth Supplement | Sigma-Aldrich | Cat# M0553-1VL |
| Middlebrook OADC Growth Supplement | Sigma-Aldrich | Cat# M0678-1VL |
| RPMI 1640 Medium | Gibico | Cat# 11875093 |
| Ketamine | Syntec | N/A |
| Xylazine | Syntec | N/A |
| CFSE | Abcam | Cat# ab113853 |
| CellTrace Violet | Thermo Fisher Scientific | Cat# C34557 |
| Fingolimod (FTY720) | Selleck Chemicals | Cat# S5002 |
| Collagenase type IV | Gibico | Cat# 17104019 |
| ACK Lysing Buffer | This paper | Made in house |
| PBS (Phosphate Buffered Saline) 1x | Corning | Cat# 21-040-CV |
| ProLong Gold antifade | Invitrogen | Cat# P36934 |
| Bovine serum albumin (BSA) | Sigma-Aldrich | Cat# A9418 |
| Fetal Bovine Serum | Gibico | Cat# A52567-01 |
| Critical commercial assays | ||
| EasySep Mouse CD4+ T cell isolation Kit | Stem Cell | Cat# 19765 |
| True Nuclear Transcription Factor Buffer Set | Biolegend | Cat# 424401 |
| BD Cytofix/Cytoperm Plus | BD Biosciences | Cat# 554715 |
| RNA isolation RNeasy Kits | QIAGEN | Cat# 74004 |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO: GSE244194 |
| Experimental models: Organisms/strains | ||
| C57BL/6 mice | Jackson Laboratory | Cat# 000664; RRID:IMSR_JAX:000664 |
| CD45.1 congenic mice | Jackson Laboratory | Cat# 002014; RRID:IMSR_JAX:002014 |
| Cd4−/− mice | Jackson Laboratory | Cat# 002663; RRID:IMSR_JAX:002663 |
| P2rx7−/− mice | Jackson Laboratory | Cat# 005576; RRID:IMSR_JAX:005576 |
| CD4-Cre | Jackson Laboratory | Cat# 022071; RRID:IMSR_JAX:022071 |
| P2rx7 flox/flox | György Haskó - Columbia University | N/A |
| Oligonucleotides | ||
| PR8-NP fwd: 5′-GATTGGTGGAATTGGACGAT-3′ | Thermo Fisher Scientific | N/A |
| PR8-NP rev: 5′-AGAGCACCATTCTCTCTATT-3′ | Thermo Fisher Scientific | N/A |
| Software and algorithms | ||
| FlowJo 10.7.1 | BD Biosciences | https://www.flowjo.com/; RRID:SCR_008520 |
| Prism 9 | Graphpad Software | https://www.graphpad.com/; RRID:SCR_002798 |
| Fiji Java 8 | ImageJ | https://github.com/imagej/ImageJ.git; RRID:SCR_002285 |
| Biorender | Biorender | https://Biorender.com; RRID:SCR_018361 |
| BGI | N/A | https://www.bgi.com/global/dr-tom/; RRID:SCR_011114 |