Table 6:
Excitation and emission information for all microscopes tested.
| Microscope | Channel | Excitation (nm) | Emission (nm) |
|---|---|---|---|
| Molecular Devices - ImageXpress Micro Confocal | Nuclei | 405 | 452/22.5 |
| ER | 470 | 520/14 | |
| RNA | 520 | 562/20 | |
| Golgi | 555 | 595/15.5 | |
| Actin | 577 | 624/20 | |
| Mitochondria | 640 | 681/12 | |
| Revvity - Opera Phenix Plus - Confocal | DNA | 375 | 457.5/22.5 |
| ER | 488 | 515/15 | |
| RNA | 488 | 532.5/17.5 | |
| AGP | 561 | 600/30 | |
| Mito | 640 | 705/55 | |
| Revvity - Opera Phenix Plus - Widefield | DNA | 375 | 457.5/22.5 |
| ER | 488 | 515/15 | |
| RNA | 488 | 600/30 | |
| AGP | 561 | 600/30 | |
| Mito | 640 | 705/55 | |
| Yokogawa - CQ1 | DNA | 405 | 447/60 |
| ER | 488 | 525/50 | |
| RNA | 488 | 617/73 | |
| AGP | 561 | 617/73 | |
| Mito | 640 | 708/75 | |
| Yokogawa - CV8000 | DNA | 405 | 445/45 |
| ER | 488 | 525/50 | |
| WGA | 561 | 600/37 | |
| Mito | 640 | 708/75 (or 676/29) | |
| RNA | 488 | 600/37 | |
| Nikon - Eclipse Ti2 | DNA | 395/25 | 460/25 |
| ER | 470/24 | 535/20 | |
| RNA/AGP | 555/25 | 620/30 | |
| Mito | 640/30 | 670/25 |