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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Nov 14;42(11):113415. doi: 10.1016/j.celrep.2023.113415

Figure 7. RBP stoichiometry in mRNPs is altered by cell growth temperature.

Figure 7.

Representative TIRF images of SNAPf-Yra1, Nab2-mNG, Npl3-mNG, and Hpr1-mNG obtained by mRNP-SiMPull from cells grown at 25°C or 37°C for 2 h. IP (Cbp80-PrA pull-down) and input (cell lysate sample) images are shown. Scale bar, 5 μm. Line graphs show uncorrected raw mean photobleaching step data from triplicate experiments for 25°C and 37°C. p values were calculated by a non-parametric Kolmogorov-Smirnoff (KS) two-sample tests. Average number (n) of spots analyzed per replicate experiment is indicated on each graph.