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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: J Mol Biol. 2023 Nov 5;435(24):168353. doi: 10.1016/j.jmb.2023.168353

Figure 6.

Figure 6.

RAD23A associates with the catalytic domain of Pol η, Pol κ and REV1. (a) The schematic illustrates the domain structure of the human Y-family polymerases. F = fingers, P = palm, T = thumb, LF = little finger, UBM = ubiquitin-binding motif, UBZ = ubiquitin-binding zinc finger. WT FLAG-RAD23A was immunoprecipitated from cells co-expressing HA-tagged catalytic domains of Pol ι, Pol η, Pol κ and REV1. The eluent and lysate were immunoblotted as indicated. (b, c, d) Immunoprecipitation of WT, V195A and F354A FLAG-RAD23A from cells co-expressing HA-tagged Pol η (b), Pol κ (c) or REV1 (d). Eluent and lysate were immunoblotted as indicated and relative binding calculated based on the ratio of HA polymerase / FLAG-RAD23A in the eluent. Bar graphs illustrate relative binding from three replicates. Error bars indicate the standard deviation. ns = not significant, * = p < 0.5, ** = p < 0.1, *** = p < 0.01, **** = p < 0.001.