Table 1:

Primary Antibodies Used to Localize the α1AR and α1AR-Containing Cells.

Antigen	Immunogen	Manufacturer Data	Dilution Used
Alpha1- adrenergic receptor (α1AR)	Synthetic peptide corresponding to residues K(339) FSREKKAAKT(349) of the third intracellular loop of human alpha-1AR; approximately 60 kDa.	ThermoFisher Scientific, Invitrogen, Rabbit Polyclonal, #PA1–047	1:1000
Tyrosine hydroxylase (TH)	Clone LNC1. Recognizes an epitope on the outside of the regulatory N-terminus; approximately 59–61 kDa. Purified from PC12 cells	MilliporeSigma, Mouse Monoclonal, #MAB318	1:750
Glutamic acid decarboxylase (GAD67)	Clone 1G10.2. Purified (by Protein A) mouse monoclonal IgG2a; approximately 67kDa.	MilliporeSigma, Mouse, Monoclonal, #MAB5406	1:1000
Vesicular glutamate transporter 1 (vGluT1)	Clone N28/9, Produced against fusion protein amino acids 493–560 of the C-terminus; approximately 52 kDa.	UC Davis/NIH NeuroMab Facility, Mouse Monoclonal, #75–066	1:1000
Vesicular glutamate transporter 2 (vGluT2)	KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat vGluT2; approximately 56 kDa.	MilliporeSigma, Guinea Pig Polyclonal, #AB2251-I	1:10,000
Glial fibrillary acidic protein (GFAP)	Clone GA5. Purified GFAP from porcine spinal cord; approximately 51 kDa.	MilliporeSigma, Chemicon, Mouse Monoclonal, #MAB360	1:3,000