Figure 6. TRPM7^wt mRNA rescues Trpm7-Em KO embryo development, but mutant versions are not as effective.

(A) Schematic of the Trpm7 mRNA rescue strategy. mRNA injections were always performed into Trpm7-Em KO zygotes, after which they were cultured in medium not supplemented with Mg²⁺.

(B–F) Bar graphs depicting rates of preimplantation embryo development of Trpm7^fl/fl and Trpm7-Em KO uninjected embryos used as controls (*p < 0.05) (B) or embryos injected with Trpm7^wt (C), Trpm7^P1040R (D), Trpm7^K1646R (E), and Trpm7^1570Stop mRNA (p > 0.05; **p < 0.01). Representative images of BLs stained with Hoechst and cell number quantification are shown to the right of each graph. Statistical comparisons were performed using Student’s t test (p > 0.05). The ‡ symbol denotes groups that did not produce BLs and were not statistically compared.