KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP | Invitrogen | Cat# G21040; RRID: AB_228307 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 | Invitrogen | Cat# A32723; AB_2535764 |
| Hoechst 33342 Solution | Thermo Scientific | 62249 |
| Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor™ 568 Conjugate | Invitrogen | L32458 |
| Monoclonal α-tubulin (Mouse monoclonal) | Sigma-Aldrich | T9026 |
| Rhodamine Phalloidin | Invitrogen | R415 |
| TRPM7 (DA5C7) | In-house made | |
| Biological samples | ||
| Mouse oocytes | Our breeding colonies. This paper | N/A |
| Mouse preimplantation embryos | This paper-Same. | N/A |
| Mouse sperm | This paper-Same. | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 3-Isobutyl-1- methylxanthine (IBMX) | Sigma-Aldrich | I5879 |
| BSA | Sigma-Aldrich | A0281-5G |
| Ethylenediaminetetraacetic acid sodium dihydrate (EDTA) | LabChem | LC137501 |
| FBS | R&D systems | S11195H |
| Hyaluronidase from bovine testes | Sigma-Aldrich | H3506 |
| Magnesium sulphate (MgSO4.7H2O) | Fisher scientific | M63-500 |
| Polyvinylpyrrolidone (PVP) | Sigma-Aldrich | PVP360 |
| Pme1 | New England Biolabs | R0560S |
| Strontium chloride hexahydrate (SrCl2) | Sigma-Aldrich | 255521 |
| Zinc Chloride | Fisher scientific | S25635A |
| Critical commercial assays | ||
| Agencourt AMPure XP kit | Beckman | A63882 |
| High pure RNA isolation kit | Roche | 12033674001 |
| Illumina DNA Prep kit | Illumina | 20018704 |
| iScript™ cDNA Synthesis Kit | BioRad | 1708890 |
| JC-1 Assay | Abcam | Ab113850 |
| Poly(A) Tailing Kit | Invitrogen | AM1350 |
| SMART-Seq v4 Ultra Low Input RNA Kit | Takara | 634894 |
| SYBR green master mix | Thermo Fisher | A46012 |
| T7 mMESSAGE mMACHINE Kit | Invitrogen | AM1344 |
| TUNEL Assay Apoptosis Detection Kit | Biotium | CF640R |
| Deposited data | ||
| Mouse eggs and early embryos RNAseq data | NCBI | GEO: GSE241487 |
| Experimental models: Organisms/strains | ||
| Mus Musculus Trpm7-floxed | A generous gift from Dr. Carmen P. Williams (NIEHS) (PMID: 30322909) | N/A |
| Mus Musculus Gdf9-cre | Jackson laboratory | 011062 |
| Mus Musculus Hspa2-cre | Jackson laboratory | 008870 |
| Mus Musculus BALB/cJ | Jackson laboratory | 000651 |
| Oligonucleotides | ||
| Refer to Table S6 for specific primers sequences. | ||
| Recombinant DNA | ||
| pcDNA6-Trpm7wt-venus | A generous gift from Dr. Vladimir Chubanov; | N/A |
| pcDNA6-Trpm7 P1040R-venus | A generous gift from Dr. Vladimir Chubanov; Ludwig-Maximilians-Universitaet Muenchen, Germany. | N/A |
| pcDNA6-Trpm7 K1646R-venus | This paper-mutations based on manuscripts published by others. | N/A |
| pcDNA6-Trpm71570Stop-venus | This paper- mutations based on manuscripts published by others. | N/A |
| pcDNA6-CALR-D1ER-KDEL | Published in previous Fissore lab paper PMID: 24101727 Original D1ER vector was a generous gift from Dr. Roger Y Tsien (PMID: 15585581) | N/A |
| Software and algorithms | ||
| Prism | GraphPad Software | Version 5.01 |
| ImageJ | NIH | N/A |
| Other | ||
| BODIPY 500/510 | Thermo Scientific | D3823 |
| DMEM | Lonza | 12-707F |
| FluoZin-3 AM | Invitrogen | F24195 |
| Fura-2 AM | Invitrogen | F1221 |
| hCG | Sigma-Aldrich | 9002-61-3 |
| Glutamax | Fisher scientific | 35-050-061 |
| Mag-Fura-2 AM | Invitrogen | M1292 |
| Pluronic F-127 (20% solution in DMSO) (Pluronic acid) | Invitrogen | P3000MP |
| PMSG | BioVendor R&D | RP1782725000 |
| Vectashield | Vector Laboratories | H-1500-10 |