. Author manuscript; available in PMC: 2024 Dec 15.

Published in final edited form as: J Mol Biol. 2023 Nov 10;435(24):168365. doi: 10.1016/j.jmb.2023.168365

Table 1.

Cryo-EM data collection statistics

Data Collection	P22 WT	P22 gp7⁻	P22 gp20⁻	P22 gp9⁻/gp26⁻	P22 gp9⁻/gp26⁻ + gp16/gp20
Facility/Microscope	PNCC/Krios	in house/Glacios	in house/Glacios	in house/Glacios	PNCC/Krios
Camera	K3	Falcon 4	Falcon 4	Falcon 4	K3
Voltage (kV)	300	200	200	200	300
Magnification	29,000 X	52,000 X	52,000 X	92,000 X	18,000 X
Electron exposure (e⁻/Å²)	30	25	25	25	30
Defocus range (μm)	−0.8 to −2.1	−1.0 to −2.4	−1.0 to −2.4	−1.0 to −1.5	−0.8 to −2.0
Pixel Size (Å)	0.80	0.95	0.95	1.50	1.28
Total movies (frames/movie)	5,331 (30)	7,669 (40)	6,343 (40)	4,440 (40)	3,819 (20)
Initial particle no.	41,597	386,885	205,120	20,717	136,921
Final particle no. (symmetry)	38,151 (C12)	51,156 (C6)	50,464 (C6)	8,159 (C6)	31,640 (C6)