TABLE 2.
Cell Mg2+ contenta
| Strain | Grown in 10 mM Mg2+b | Mgt protein expressed | Mg2+ content ± SD (nmol/OD600) |
|---|---|---|---|
| MM281 | + | None | 1,397 ± 117 |
| MM1490 | + | MgtB | 1,916 ± 73 |
| MM1665 | + | None | 1,253 ± 70 |
| MM1665c | − | MgtC | 904 ± 49 |
Mg2+ content was measured by atomic absorption as described in Materials and Methods in four individual cell samples. A second experiment gave comparable results.
+, grown in the presence of 10 mM Mg2+; −, grown in the absence of Mg2+.
Cells were starved for Mg2+ for 41 h.