Table I. HMGN proteins increase the intranuclear mobility of H10–GFP.
| Experiment | t40 |
Recovery after 23 s |
|||
|---|---|---|---|---|---|
| Time (s) ± SD | t-test | % ± SD | t-test | ||
| H10–GFP | Mixed chromatin domains | 14.7 ± 4.1 | P < 0.005 | 46.7 ± 5.6 | P < 0.005 |
| H10–GFP + H1 | 5.7 ± 2.1 | 66.3 ± 8.5 | |||
| H10–GFP | Mixed chromatin domains | 13.7 ± 4.1 | P < 0.06 | 46.7 ± 5.6 | P < 0.02 |
| H10–GFP + HMGN1 | 9.3 ± 5.1 | 58.9 ± 10.1 | |||
| H10–GFP | Mixed chromatin domains | 12.8 ± 3.1 | P < 0.005 | 50.7 ± 6.1 | P < 0.005 |
| H10–GFP + HMGN2 | 6.0 ± 1.5 | 61.0 ± 3.6 | |||
| H10–GFP | Mixed chromatin domains | 12.5 ± 1.8 | P > 0.6 | 46.9 ± 5.0 | P > 0.6 |
| H10–GFP + H2B | 13.2 ± 3.8 | 45.8 ± 3.8 | |||
| H10–GFP | Mixed chromatin domains | 15.4 ± 3.0 | P > 0.2 | 46.0 ± 3.9 | P > 0.6 |
| H10–GFP + HMGN1-S20,24E | 13.6 ± 3.3 | 47.1 ± 6.5 | |||
| H10–GFP | Mixed chromatin domains | 12.3 ± 3.8 | P > 0.6 | 46.9 ± 6.5 | P > 0.8 |
| H10–GFP + HMGN1 (1–73) | 13.2 ± 3.4 | 46.4 ± 4.7 | |||
| H10–GFP | Euchromatin | 10.2 ± 3.5 | P < 0.005 | 55.9 ± 7.3 | P < 0.005 |
| H10–GFP + HMGN1 | 4.2 ± 1.4 | 69.0 ± 9.9 | |||
| H10–GFP | Euchromatin | 10.6 ± 2.7 | P < 0.005 | 52.2 ± 3.4 | P < 0.005 |
| H10–GFP + HMGN2 | 2.6 ± 0.6 | 79.0 ± 4.1 | |||
| H10–GFP | Heterochromatin | > 23 s | n.a. | 35.1 ± 5.8 | P < 0.005 |
| H10–GFP + HMGN1 | 10.5 ± 3.8 | 53.7 ± 8.2 | |||
| H10–GFP | Heterochromatin | > 23 s | n.a. | 36.3 ± 3.2 | P < 0.005 |
| H10–GFP + HMGN2 | 8.2 ± 1.8 | 55.9 ± 6.3 | |||
The time required to reach 40% (t40) of the pre-bleach fluorescence intensity was determined from each curve. The recoveries at 23 s are means of the last five images of each recovery curve (last 0.6 s). The results are from recovery curves of at least eight cells. The statistical significance of the differences of t40 and total recovery relative to controls was determined by Student’s t-test.