Skip to main content
. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Oct 18;42(10):113248. doi: 10.1016/j.celrep.2023.113248

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies

Mouse-Anti-ST2 IgG Invitrogen Cat #MA5–37747; RRID:AB_2897671
Mouse-Anti-HisTag antibody His.H8 Invitrogen Cat #MA1–21315; RRID:AB_557403
HRP goat anti-mouse IgG SouthernBiotech Cat #1030–05; RRID:AB_2619742
HRP goat anti-human IgG SouthernBiotech Cat #2040–05; RRID:AB_2795644
HRP goat anti-rabbit IgG Abcam Cat #ab97080; RRID:AB_10679808

Bacterial and virus strains

SARS-CoV-2 virus, Isolate USA-WA1/2020 BEI Resources Cat #NR-52281
SARS-CoV-2 nanoLuc virus Hou et al.37 N/A
SARS-CoV nanoLuc virus Sheahan et al.38 N/A
WIV1-CoV nanoLuc virus Menachery et al.20 N/A
SARS-CoV mouse-adapted virus MA15 Roberts et al.22 N/A
MERS-CoV mouse-adapted virus m35c4 Douglas et al.24 N/A

Biological samples

Plasma, nasal turbinates, and lungs from mice This paper N/A

Chemicals, peptides, and recombinant proteins

SureBlue Reserve tetramethylbenzidine substrate KPL Cat #5120–0081
Luciferase Cell Culture Lysis 5x Reagent Promega Cat# E1531
Background Reducing Antibody Diluent Agilent Cat# S3022
PowerVision Poly-HRP anti-Rabbit IgG IHC Detection Systems Leica Cat# PV6121
Human ACE2 soluble protein Edwards et al.39 N/A
SARS-CoV-2 Spike S2 ECD This paper N/A
SARS-CoV-2 Spike RBD This paper N/A
MERS-CoV Spike ECD This paper N/A
MERS-CoV Spike RBD This paper N/A
RsSHC014 Spike RBD This paper N/A
RsSHC014 Spike ECD This paper N/A
H. pylori Ferritin This paper N/A
Sortase A This paper N/A
CCoV-HuPn RBD This paper N/A
hCoV-OC43 RBD This paper N/A
WIV-1 RBD This paper N/A
SARS-CoV GZ02 RBD This paper N/A
BatCoV-ZC45 RBD This paper N/A
PCoV-GXP4L RBD This paper N/A
BatCoV-BANAL-236 RBD This paper N/A
BatCoV-NL140422 RBD This paper N/A
BatCoV-HKU4 RBD This paper N/A
BatCoV-HKU5 RBD This paper N/A
BatCoV-BtKY06 RBD This paper N/A
PdCoV-Haiti RBD This paper N/A

Critical commercial assays

Britelite Luminescence Reporter Gene Assay System PerkinElmer Life Sciences Cat #6066761
Nano-Glo Luciferase Assay System Promega Cat #N1150

Experimental models: Cell lines

Vero E6 ATCC Cat# CRL-1586
Freestyle 293F ThermoFisher Scientific Cat# R79007

Experimental models: Organisms/strains

BALB/c mouse Envigo N/A
288/330 hDPP4 transgenic mice Cockrell et al.23 N/A

Recombinant DNA

HV1302284 (H. pylori Ferritin) Saunders et al.7 N/A
HV1302277 (Sortase A) Saunders et al.7 N/A
HV1302964 (SARS-CoV-2 RBD) Saunders et al.7 N/A
HV1302315 (BatCoV-RsSHC014 RBD) This paper N/A
HV1301961_D614G (SARS-CoV-2 D614G S2P Spike) This paper N/A
HV1302109 (BatCoV-RsSHC014 S2P Spike) This paper N/A
HV1302103 (SARS-CoV Tor2 S2P Spike) This paper N/A
HV1302112 (MERS-CoV S2P Spike) This paper N/A
HV1302631 (CCoV-HuPn RBD) This paper N/A
HV1302784 (hCoV-OC43 RBD) This paper N/A
HV1302780 (WIV-1 RBD) This paper N/A
HV1302316 (SARS-CoV GZ02 RBD) This paper N/A
HV1303162 (BatCoV-ZC45 RBD) This paper N/A
HV1302314 (PCoV-GXP4L RBD) This paper N/A
HV1303029 (BatCoV-BANAL-236 RBD) This paper N/A
HV1302313 (MERS-CoV RBD) This paper N/A
HV1302782 (BatCoV-NL140422 RBD) This paper N/A
HV1303455 (BatCoV-HKU4 RBD) This paper N/A
HV1303456 (BatCoV-HKU5 RBD) This paper N/A
HV1303169 (BatCoV-BtKY06 RBD) This paper N/A
HV1302636 (PdCoV-Haiti RBD) This paper N/A

Software and algorithms

GraphPad Prism v 9 GraphPad Software Inc https://www.graphpad.com/scientific-software/prism/
PyMol The PyMOL Molecular Graphics System (Schro¨ dinger, 2015). https://www.pymol.org/2/
SoftMax Pro Molecular Devices https://www.moleculardevices.com/
Adobe Illustrator 2020 Adobe N/A
Relion Scheres,40 Scheres et al.41 Version 3.1
UCSF Chimera Pettersen et al.42 http://www.cgl.ucsf.edu/chimera/
Chimera X Goddard et al.43 https://www.rbvi.ucsf.edu/chimerax/
Adobe Photoshop CC 2019 Adobe N/A