Antibodies |
|
Mouse-Anti-ST2 IgG |
Invitrogen |
Cat #MA5–37747; RRID:AB_2897671 |
Mouse-Anti-HisTag antibody His.H8 |
Invitrogen |
Cat #MA1–21315; RRID:AB_557403 |
HRP goat anti-mouse IgG |
SouthernBiotech |
Cat #1030–05; RRID:AB_2619742 |
HRP goat anti-human IgG |
SouthernBiotech |
Cat #2040–05; RRID:AB_2795644 |
HRP goat anti-rabbit IgG |
Abcam |
Cat #ab97080; RRID:AB_10679808 |
|
Bacterial and virus strains |
|
SARS-CoV-2 virus, Isolate USA-WA1/2020 |
BEI Resources |
Cat #NR-52281 |
SARS-CoV-2 nanoLuc virus |
Hou et al.37
|
N/A |
SARS-CoV nanoLuc virus |
Sheahan et al.38
|
N/A |
WIV1-CoV nanoLuc virus |
Menachery et al.20
|
N/A |
SARS-CoV mouse-adapted virus MA15 |
Roberts et al.22
|
N/A |
MERS-CoV mouse-adapted virus m35c4 |
Douglas et al.24
|
N/A |
|
Biological samples |
|
Plasma, nasal turbinates, and lungs from mice |
This paper |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
SureBlue Reserve tetramethylbenzidine substrate |
KPL |
Cat #5120–0081 |
Luciferase Cell Culture Lysis 5x Reagent |
Promega |
Cat# E1531 |
Background Reducing Antibody Diluent |
Agilent |
Cat# S3022 |
PowerVision Poly-HRP anti-Rabbit IgG IHC Detection Systems |
Leica |
Cat# PV6121 |
Human ACE2 soluble protein |
Edwards et al.39
|
N/A |
SARS-CoV-2 Spike S2 ECD |
This paper |
N/A |
SARS-CoV-2 Spike RBD |
This paper |
N/A |
MERS-CoV Spike ECD |
This paper |
N/A |
MERS-CoV Spike RBD |
This paper |
N/A |
RsSHC014 Spike RBD |
This paper |
N/A |
RsSHC014 Spike ECD |
This paper |
N/A |
H. pylori Ferritin |
This paper |
N/A |
Sortase A |
This paper |
N/A |
CCoV-HuPn RBD |
This paper |
N/A |
hCoV-OC43 RBD |
This paper |
N/A |
WIV-1 RBD |
This paper |
N/A |
SARS-CoV GZ02 RBD |
This paper |
N/A |
BatCoV-ZC45 RBD |
This paper |
N/A |
PCoV-GXP4L RBD |
This paper |
N/A |
BatCoV-BANAL-236 RBD |
This paper |
N/A |
BatCoV-NL140422 RBD |
This paper |
N/A |
BatCoV-HKU4 RBD |
This paper |
N/A |
BatCoV-HKU5 RBD |
This paper |
N/A |
BatCoV-BtKY06 RBD |
This paper |
N/A |
PdCoV-Haiti RBD |
This paper |
N/A |
|
Critical commercial assays |
|
Britelite Luminescence Reporter Gene Assay System |
PerkinElmer Life Sciences |
Cat #6066761 |
Nano-Glo Luciferase Assay System |
Promega |
Cat #N1150 |
|
Experimental models: Cell lines |
|
Vero E6 |
ATCC |
Cat# CRL-1586 |
Freestyle 293F |
ThermoFisher Scientific |
Cat# R79007
|
|
Experimental models: Organisms/strains |
|
BALB/c mouse |
Envigo |
N/A |
288/330 hDPP4 transgenic mice |
Cockrell et al.23
|
N/A |
|
Recombinant DNA |
|
HV1302284 (H. pylori Ferritin) |
Saunders et al.7
|
N/A |
HV1302277 (Sortase A) |
Saunders et al.7
|
N/A |
HV1302964 (SARS-CoV-2 RBD) |
Saunders et al.7
|
N/A |
HV1302315 (BatCoV-RsSHC014 RBD) |
This paper |
N/A |
HV1301961_D614G (SARS-CoV-2 D614G S2P Spike) |
This paper |
N/A |
HV1302109 (BatCoV-RsSHC014 S2P Spike) |
This paper |
N/A |
HV1302103 (SARS-CoV Tor2 S2P Spike) |
This paper |
N/A |
HV1302112 (MERS-CoV S2P Spike) |
This paper |
N/A |
HV1302631 (CCoV-HuPn RBD) |
This paper |
N/A |
HV1302784 (hCoV-OC43 RBD) |
This paper |
N/A |
HV1302780 (WIV-1 RBD) |
This paper |
N/A |
HV1302316 (SARS-CoV GZ02 RBD) |
This paper |
N/A |
HV1303162 (BatCoV-ZC45 RBD) |
This paper |
N/A |
HV1302314 (PCoV-GXP4L RBD) |
This paper |
N/A |
HV1303029 (BatCoV-BANAL-236 RBD) |
This paper |
N/A |
HV1302313 (MERS-CoV RBD) |
This paper |
N/A |
HV1302782 (BatCoV-NL140422 RBD) |
This paper |
N/A |
HV1303455 (BatCoV-HKU4 RBD) |
This paper |
N/A |
HV1303456 (BatCoV-HKU5 RBD) |
This paper |
N/A |
HV1303169 (BatCoV-BtKY06 RBD) |
This paper |
N/A |
HV1302636 (PdCoV-Haiti RBD) |
This paper |
N/A |
|
Software and algorithms |
|
GraphPad Prism v 9 |
GraphPad Software Inc |
https://www.graphpad.com/scientific-software/prism/
|
PyMol |
The PyMOL Molecular Graphics System (Schro¨ dinger, 2015). |
https://www.pymol.org/2/
|
SoftMax Pro |
Molecular Devices |
https://www.moleculardevices.com/
|
Adobe Illustrator 2020 |
Adobe |
N/A |
Relion |
Scheres,40 Scheres et al.41
|
Version 3.1 |
UCSF Chimera |
Pettersen et al.42
|
http://www.cgl.ucsf.edu/chimera/
|
Chimera X |
Goddard et al.43
|
https://www.rbvi.ucsf.edu/chimerax/
|
Adobe Photoshop CC 2019 |
Adobe |
N/A |