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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell. 2023 Oct 20;186(23):5041–5053.e19. doi: 10.1016/j.cell.2023.09.017

Figure 5. Characterization of mutant Tekt 5 −/− sperm.

Figure 5.

(A) The percentages of motile sperm from wild-type and Tekt 5 knockout mice (> 200 cells were counted for each mouse and three knockout −/− mice and two wild-type mice were analyzed, the pool percentage and 95% Confidence Intervals (Wilson/Brown method) were shown). (B) The percentages of bent sperm from wild-type and Tekt 5 knockout mice (> 200 cells were counted for each mouse and three knockout −/− mice and two wild-type mice were analyzed, the pool percentage and 95% Confidence Intervals by Wilson/Brown method were shown). Two examples of bent sperm are shown. (C) An overlay of wild-type models with the densities of Tekt 5 −/− sperm around the slanted bundles. The continuous 3-helix bundle assigned as Tektin 5 (high occupancies) and slanted helical bundles (low occupancies) are shown. The densities corresponding to the DUSP proteins are barely resolved. Note there are substantially less densities for these models compared to Figure 3C. (D) An overlay of wildtype models with the densities of Tekt 5 −/− sperm around the curved bundles. The occupancies of the curved bundles are lower than the other MIPs and tubulins. Note there are substantially less densities for these models compared to Figure 3D. (E) The two broken 3-helix bundles have lower occupancies compared to the surrounding MIPs and tubulins. Note there are substantially less densities for these models compared to Figure 3A.