Figure 9:

Mutation of amino acids in the PAPS binding (H40I) or catalytic (H96F) domains tend to attenuate effects of wild type XB5850668.L gain-of-function on otic vesicle gene domains.

Percentage of embryos in which an OV gene expression domain was reduced (dark blue), broader (medium blue) or did not change (light blue) after injections of mRNAs (100 pg) encoding wild type (wt) or mutated versions (K40I, H96F, double = K40I+H96F) of XB5850668.L. The number of embryos analyzed for each gene is inside the bars. * indicates mutant mRNA phenotype frequencies that were significantly different from wt at the p<0.05 level (Chi square test).

A: six1 OV expression domain changes.

B: irx1 OV expression domain changes.

C: sox9 OV expression domain changes.

D: eya2 OV expression domain changes.

E: pax2 OV expression domain changes.

F: dlx5 OV expression domain changes.

G: tbx1 OV expression domain changes.