KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
|---|---|---|---|---|
| Antibodies | ||||
| Rabbit monoclonal antibody to cleaved PARP | Cell Signaling Technology | CAT#5625S; RRID: AB_10699459 | ||
| Rabbit monoclonal antibody to Ezrin | Cell Signaling Technology | CAT#3145S; RRID: AB_3065058 | ||
| Mouse monoclonal antibody to CD44 | Cell Signaling Technology | CAT#3570S; RRID: AB_2076465 | ||
| Mouse anti-mitochondria antibody [113-1] | Abcam | CAT#ab92924; RRID: AB_94052 | ||
| Alexa Fluor™ 647 Phalloidin (Actin) | Invitrogen™ | CAT#A22287 | ||
| Hoechst 33342 (Nuclei) | Thermo Scientific | CAT#62249 | ||
| Donkey anti-mouse IgG secondary antibody, Alexa Fluor 555 | Fisher scientific | CAT#A31570 | ||
| Donkey anti-rabbit IgG secondary antibody, Alexa Fluor 647 | Fisher scientific | CAT#A31573 | ||
| Bacterial and virus strains | ||||
| pLV-FireflyLuc-GFP | UCLA Vector Core | N/A | ||
| Chemicals, peptides, and recombinant proteins | ||||
| Sodium Hyaluronate (MW = 700kDa) | LifeCore Biomedical | CAT# HA700k-5 | ||
| 8-arm norbornene functionalized polyethylene glycol (MW = 20kDa) | JenKem | CAT# A10037-1 | ||
| 4-arm thiol functionalized polyethylene glycol (MW = 20kDa) | Laysan Bio | CAT# 4arm-PEG-SH-20K-1g | ||
| Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, ≥95% | Sigma Aldrich | CAT# 900889-1G | ||
| RGD peptide (Sequence: GCGYGRGDSPG) | Genscript | Custom order | ||
| Critical commercial assays | ||||
| EdU proliferation kit | Abcam | CAT# ab222421 | ||
| Deposited data | ||||
| RNA-sequencing data | This paper | GEO accession number GSE239610 | ||
| Experimental models: Cell lines | ||||
| Patient-derived gliomasphere lines | Kornblum lab (UCLA) | N/A | ||
| Experimental models: Organisms/strains | ||||
| NOD-scid IL2Rgnull” (NOD-Scid gamma) (Female) | The Jackson laboratory | Strain #:005557 | ||
| Oligonucleotides | ||||
| HYAL1 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn | Thermofisher | Hs00201046_m1 | ||
| HYAL2 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs00186841_m1 | ||
| HYAL3 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs00185910_m1 | ||
| MMP2 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs01548727_m1 | ||
| MMP9 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs00957562_m1 | ||
| GAPDH TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs02758991_g1 | ||
| TGFB1TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs00998133_m1 | ||
| TIMP1 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs01092512_g1 | ||
| CHI3L1 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | Hs01072228_m1 | ||
| OLIG2 TaqMan® Gene Expression Assays, Dye: FAM-MGB-small 250rxn cat#4331182 | Thermofisher | HS0300164_s1 | ||
| Software and algorithms | ||||
| Ingenuity Pathway Analysis (IPA) | Qiagen | www.ingenuity.com | ||
| Prism 10 | Graphpad | https://www.graphpad.com/features | ||
| ImageJ | National Institute of Health | https://imagej.nih.gov/ij/ | ||
| JPKSPM AFM data processing | Bruker | N/A | ||
| Mitometer | Digman lab (UCI) | https://github.com/aelefebv/Mitometer | ||
| Biorender | Biorender | https://www.biorender.com/ | ||