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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Oct 8;42(10):113223. doi: 10.1016/j.celrep.2023.113223

Figure 7. SUGP1’s G-patch domain is sufficient to bind DHX15 but not to recruit it to nuclear foci.

Figure 7.

(A) Protein blots and quantification of biotinylated proteins labeled by FL versus truncated SUGP1-DHX15 interaction-reconstituted split-APEX activity. Ponceau S protein stain, loading control for total protein; streptavidin IRDye, detection of biotinylated proteins; FRB-nls, FRB control protein fused with an SV40 NLS; ΔGp, G-patch domain truncation; Gp-nls, G-patch domain alone fused with an SV40 NLS.

(B) Fluorescent microscopy images of biotinylation signals (streptavidin, magenta), antibodies detecting HA-tagged SUGP1-EX, FL versus Gp-nls (HA, red), FLAG-tagged AP-DHX15 (FLAG, green), nuclear DNA dye (DAPI, blue), and merged channels.

(C) A sampling-and-recruitment model of DHX15-SUGP1 interaction during early-splicing QC.