FIG. 5.

RPA of IFN-γ mRNA from total LNs of individual C3H mice and BALB/c mice 2 days postinfection with L. major. Representative RPAs for a single C3H mouse and BALB/c mouse are shown on the left and right, respectively. Cyc, cyclophilin. The averages and standard deviations for IFN-γ mRNA quantitation in relative units are shown for one experiment, which included three C3H mice. U, uninfected; I, infected; I IgG, infected plus treatment with control antibody; I αIL-12, infected plus treatment with anti-IL-12 antibodies. These results are representative of three experiments. IFN-γ mRNA quantitation for BALB/c mice is identical to that for C3H mice. U IL-12, uninfected plus treatment with IL-12; I IL-12, infected plus treatment with IL-12. The IFN-γ mRNA relative-unit value for infected mice treated with IL-12 is the average of results for two individual mice. Infection and treatment are described in Materials and Methods.