FIG. 7.

IL-12 receptor β1 and β2 subunit mRNA expression and IL-12 responsiveness of LN cells during the early immune response to L. major infection in C3H and BALB/c mice. (A) Representative RPA of IL-12Rβ1 and -β2 mRNA from LNs of individual C3H or BALB/c mice 2 days after infection with L. major. The RPA shown is representative of samples from five or six individual mice per group. U, uninfected; I, infected. Also shown are the relative units of β1 or β2 mRNA at 2 days postinfection in C3H and BALB/c mice. Each column shows the means and standard deviations for five or six mice. (B) Lymphocytes isolated from the LNs of C3H or BALB/c mice infected 2 days previously were incubated with IL-12 (1 ng/ml) for 24 h. The supernatants were then harvested, and IFN-γ levels were measured by ELISA to assess IL-12 responsiveness. The results are representative of two experiments with three individual mice each.