Fig. 3. Distribution of run-on values along a given gene sequence. Oligonucleotides complementary to the atp1 and rrn18 transcripts (horizontal gray arrows) were spotted on nylon filters and hybridized with mitochondrial run-on transcripts (central part of the figure) obtained by incorporating [α-³²P]UTP. The intensity of the signals was quantified and its variation from 5′ to 3′ is indicated in the upper part of the figure.